Direct isolation of specific RNA-interacting proteins using a novel affinity medium

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Direct isolation of specific RNA-interacting proteins using a novel affinity medium

Isolation of proteins that specifically interact with a given RNA or RNA regulation element is essential for studies on the molecular mechanisms of gene expression. Here, a novel method for direct isolation of such interacting proteins is described. It uses an affinity medium that consists of an interacting RNA with an artificially added 'tail', which is annealed to one end of a DNA 'arm', the ...

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ژورنال

عنوان ژورنال: Nucleic Acids Research

سال: 2005

ISSN: 0305-1048,1362-4962

DOI: 10.1093/nar/gni133